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. 2023 May 1;5(2):zcad018. doi: 10.1093/narcan/zcad018

Figure 4.

Figure 4.

Z56 and Z99 interfere with RAD52-MUS81 function at stalled DNA replication forks. (A) Cartoon representation of fork cleavage by the RAD52-MUS81 axes. The presence of the DSBs can be detected using comet assay (B–D). (B) Evaluation of DSBs using Neutral Comet Assay. Cells were treated as indicated. Graph shows the mean of tail moment as mean ± SE (ns = not significant; ***P< 0.001; ****P < 0.0001; Mann–Whitney test). When indicated, cells were treated with inhibitors for 30 min before HU exposure. (C) Analysis of DSBs in MRC5 SV40 and MRC5 shRAD52 after HU exposure in presence or absence of RAD52 inhibitors (30 min before HU treatment). Graph shows the mean of tail moment ± SE (ns = not significant; *P< 0.1; ****P < 0.0001; Mann–Whitney test). (D) After 48 h of doxycycline induction cells were treated to perform neutral comet assay. Western blot shows BRCA2 level after doxycycline induction. LAMIN B1 was used as a loading control. Graph shows the mean of tail moment ± SE (****P < 0.0001; Mann–Whitney test).