FIG. 3.

Demonstration of the presence of allele-specific and conserved epitopes in rF-seg-INT and rINT-C-seg recombinant antigens. ELISA analysis of serum IgG in a rabbit immunized with rF-seg-INT (×, preimmunization; □, postimmunization) and in a rabbit immunized with rINT-C-seg (○, preimmunization; ●, postimmunization) is shown. (A) ELISA plate coated with rF-seg-INT; (B) ELISA plate coated with rINT-C-seg. Antigens were applied at 0.5 μg ml⁻¹. Assay details are given in Materials and Methods.