FIGURE 4.

MCAM negatively regulates TEM of brain metastatic variant of breast cancer (A) MDA cells were transiently transfected with siRNA targeting CD146 (si146) or a scrambled control siRNA (Scr), CTG labelled, and seeded to the upper chamber of a Boyden transwell chamber in the presence of HUVEC monolayers. For the No EC condition, MDA cells were seeded to empty transwells only. CTG cells that had migrated to the underside of the transwell filter were imaged (as shown) and quantified 18 h post-seeding, as shown in the bar diagrams. Scale bar: 200 μm. Data were derived from three independent experiments. Error bars indicate S.E.M. Analysis was performed using an unpaired T-test: *p < 0.05; ns, not significant. (B) As in (A) but using MDA-BrM cells. Scale bar: 1,000 μm. Data were derived from three independent experiments. Error bars indicate S.E.M. Analysis was performed using an unpaired T-test; *p < 0.05; **p < 0.01.