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. Author manuscript; available in PMC: 2024 Mar 15.
Published in final edited form as: Neuron. 2023 Jan 27;111(6):787–796.e4. doi: 10.1016/j.neuron.2022.12.011

Figure 1. CRISPR mutagenesis yields multiple null alleles of Oxtr in prairie voles.

Figure 1.

A. Schematic of CRISPR-based targeting to generate Oxtr mutant prairie voles. Single cell embryos injected with Cas9-sgRNA ribonucleoprotein were cultured (1) to the blastocyst stage and transferred (2) to pseudopregnant recipient females who carried the embryos to term (3).

B. Schematic (top) of Oxtr locus encompassing first two exons. DNA sequence of WT and targeted Oxtr alleles. Dash/missing nucleotide represents a deletion (Oxtr4, Oxtr5) and red highlighted “C” (Oxtr1) is an insertion. PAM, protospacer adjacent motif.

C. Predicted amino acid sequence of WT Oxtr, Oxtr1, Oxtr4, and Oxtr5 (only first 100 amino acids shown).

See also Figure S1.