FIG. 1.

Incubation with recombinant bovine IL-1β upregulates β₂-integrin expression on bovine PMNs. (A and B) Freshly isolated bovine peripheral blood PMNs (10⁶ cells/ml) were incubated with IL-1β (50 ng) for 15 min at 37°C (open trace) or with medium (solid trace) before incubation (40 min at 4°C) with anti-β₂-integrin MAb BAT75A (50-μg/ml final concentration) (A) or CA1.4E9 (50-μg/ml final concentration) (B). The cells were then washed, incubated with an FITC-labeled second antibody, and analyzed by flow cytometry (10,000 cells were scored for green fluorescence). Panels A and B represent a single representative experiment. (C and D) Mean (± standard error of the mean) percent positive cells for five independent experiments (P < 0.01 compared with unstimulated cells [Con]).