Figure 4.
Pretreatment with intravenous 3-deacyl 6-acyl phosphorylated hexaacyl disaccharide (PHAD) in mice that underwent bilateral ischemia-reperfusion injury-induced acute kidney injury. Mice were pretreated with intravenous PHAD at 200 µg/mouse or vehicle control 48 and 24 h prior to undergoing bilateral renal pedicle clamping for 24 min. Quantitative RT-PCR for renal expression of heme oxygenase-1 (Ho-1; A), interferon regulatory factor-1 (Irf1; B), chemokine (C-C motif) ligand 2 (Ccl2; C), and chemokine (C-C motif) ligand 3 (Ccl3; D) day 3 after injury. GAPDH was used as the internal control for quantitative RT-PCR, and change in threshold cycle (ΔCt) values is graphed relative to the vehicle-treated group. Individual data points and medians are shown, and P values are shown from a Mann-Whitney U test used to compare groups. n = 6 or 7.