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. 2023 Mar 17;324(6):H762–H775. doi: 10.1152/ajpheart.00638.2022

Figure 6.

Figure 6.

Impact of ovarian hormones loss on diabetic phenotype, SBP and soluble prorenin receptor (sPRR) components, tissue weights, and plasma estradiol. Body weight (A) and fasting glycemia (B) were elevated in ovariectomy (OVX)-high-fat diet (HFD) compared with OVX- normal fat diet (NFD) female mice, paired t test, ***P < 0.001. Fasting glycemia (C) at 30, 60, and 90 min in response to a glucose overload was elevated in HFD group, two-way ANOVA results listed on graph with Sidak’s post hoc tests, *P < 0.05, **P < 0.01, ****P < 0.0001. The area under the curve (AUC) for intraperitoneal glucose tolerance test (ipGTT) (D) was elevated in the OVX-HFD group, t test, ****P < 0.0001. No changes were observed in plasma insulin levels, two-way ANOVA (E). SBP was higher in OVX-HFD females starting at 40 wk of HFD (8 wk post-OVX) (F). Two-way ANOVA results listed on graph with Sidak’s post hoc tests, *P < 0.05. Final SBP was higher in OVX-HFD mice, unpaired t test (G). Total plasma prorenin/renin (H) and sPRR (J) were elevated in OVX-HFD, unpaired t test, *P < 0.05, **P < 0.01. Plasma ANG II levels were not elevated in female mice, unpaired t test (I). Vascular contractility in response to PGF2α was not impacted by HFD (K). Vasocontraction to phenylephrine (PE) was decreased in the OVX-HFD group (L). ACh relaxation in PGF2α preconstricted rings was significantly reduced in OVX-NFD vessels (M). Endothelium-independent relaxation assessed using sodium nitroprusside (SNP) was unchanged by HFD, all two-way ANOVA with results listed on graph and Sidak’s post hoc tests, *P < 0.05 (N). Aortic reactive oxygen species (ROS) production was not impacted by HFD (O). Heart and kidneys were increased in OVX-HFD (P), and no changes were observed in liver and pancreas weight (Q). Paired t tests, *P < 0.05, ***P < 0.0001. Visceral, subcutaneous, and brown fat tissue weights were significantly higher in female mice fed a HFD than in those on NFD (R). ns, not significant. Two-way ANOVA with results listed on graph and Sidak’s post hoc tests. **P < 0.001. Plasma estradiol concentrations were reduced after 12 wk of OVX in all groups tissue (S). Data are represented as means ± SD.