Left ventricular end-diastolic pressure (LVEDP) from “baseline” to “end point” in three groups: group 1, n = 4 (A); group 2, n = 8 (B); and group 3, n = 8 (C). D: means ± SD data of individual groups. Two-tailed paired t tests were used to compare data between 2 time points (baseline vs. end point) within the same group (A, B, and C). A two-way mixed ANOVA model for D was conducted to analyze the time effect (P < 0.0001), groups effect (P = 0.0005), and time/groups interaction effect (P = 0.0009) on LVEDP. Post hoc tests with Bonferroni correction are shown at end points between the three groups.