Table A2. List of reagents and materials used in RT-PCR.
| Name | Company | Catalogue no. |
|---|---|---|
| Taq PCR Master-mix kit (2×) | Qiagen/ Thermo Scientific | 201445/K0171 |
| Nuclease – free water | ----- | ---- |
| 100 bp ladder | Fermentas | SM0241 |
| Forward primer | Sigma Genosys | --- |
| Reverse primer | Sigma Genosys | --- |
| cDNA | --- | --- |
| Autoclaved H2O | ---- | --- |
| 0.2-μL thin-walled PCR tubes | Axygen | 321-01-051 |
| Sterile pipette tips | Tarsons | 521000,521010 |
| Cooled PCR rack | Tarsons | 240060 |
| Ice bucket plus ice | Sigma | Z407925 |
| Glass bottle | Borosil | 6254C |
| Powder-free gloves | Axygen | 120323 |
All instruments were calibrated as per the standard guidelines. Positive control and negative control (Plasmid controls or known positive and negative cases) were used