Table A3. Preparation of PCR mix.
| Reagents | Volume | Volume | Volume | Volume |
|---|---|---|---|---|
| 2× Master mix | 5 μL | 5 μL | 5 μL | 5 μL |
| 5 pM PAX3 primer (Forward) | 0.5 μL | 0.5 μL | 0.5 μL | 0.5 μL |
| 5 pM FKHR primer (Reverse) | 0.5 μL | 0.5 μL | 0.5 μL | 0.5 μL |
| 10 pM PAX7 primer (Forward) | 0.5 μL | 0.5 μL | 0.5 μL | 0.5 μL |
| 10 pM FKHR primer (Reverse) | 0.5 μL | 0.5 μL | 0.5 μL | 0.5 μL |
| cDNA template | 1 μL | -- | -- | -- |
| Positive control | -- | 1 μL | -- | -- |
| Negative control | -- | -- | 1 μL | -- |
| H2O | 3 μL | 3 μL | 3 μL | 4 μL |
| Total | 10 μL | 10 μL | 10 μL | 10 μL |
| Sample no. | Test | Positive control | Negative control | Reagent control |
| Tube no. | 1 | 2 | 3 | 4 |