Table 4.
Reaction procedures and conditions.
| Cycle steps | Temperature | Time | Cycle number |
|---|---|---|---|
| Premutability | 95°C | 5 min | 1 |
| Transgender | 95°C | 10 sec | 40 |
| Annealing/extension | 60°C | 30 sec | |
| Dissolution curve | Instrument default settings | 1 | |
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