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. 2023 Feb 3;146(5):1979–1992. doi: 10.1093/brain/awad031

Figure 3.

Figure 3

Fewer mature oligodendrocytes in lysolecithin-induced lesions in PPMS CSF-injected mice. (A and C) Representative images of the dorsal column in cervical spinal cords stained with NG2/Ki67 (A) and APC/Olig2 (C) at 12 days post lysolecithin injection. Mice received intrathecal injections of saline or CSF from HC (n = 3), PPMS (n = 8), RRMS (n = 7) or SPMS patients (n = 6) at 5 days post lysolecithin injection (Supplementary Table 1). Saline (n = 31 mice), HC (n = 13 mice), PPMS (n = 36 mice), RRMS (n = 23 mice), SPMS (n = 16 mice). Scale bars = 100 µm; inset = 50 µm. (B) Quantification of the number of NG2+Ki67+ proliferating OPCs throughout the lesion. (D) Quantification of the number of APC+Olig2+ mature oligodendrocytes throughout the lesion. Data plotted as mean ± SEM. Each point represents an individual mouse. One-way ANOVA with Bonferroni’s test (B and D). *P < 0.05.