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. 2023 Feb 3;146(5):1979–1992. doi: 10.1093/brain/awad031

Figure 5.

Figure 5

Depletion of IgG from PPMS CSF eliminates its pathogenic capacity. (A) Representative Coomassie Blue staining of a PPMS CSF sample, IgG-depleted PPMS CSF and the IgG eluted from the depletion. Individual PPMS CSF samples (n = 3) were incubated with DynabeadsTM Protein A to deplete IgGs (Supplementary Table 2). Boxes indicate presumptive positions of IgG heavy and light chains. (B) Motor deficit scores at 1 DPI. Saline (n = 9 mice), PPMS CSF (n = 9 mice), IgG-depleted PPMS CSF (n = 9 mice). (C) Forelimb grip strength force at 1 DPI normalized to pre-surgery baseline force. (D) Representative GFAP immunostaining images and quantification of mean fluorescence intensity in the cervical spinal cord dorsal column at 1 DPI. (E) Representative SMI-32 immunostaining images and quantification of mean fluorescence intensity in the cervical spinal cord dorsal column at 1 DPI. Data plotted as mean ± SEM. Each point represents an individual mouse. One-way ANOVA with Bonferroni’s test (B–E). ****P < 0.0001, **P < 0.01, *P < 0.05. Scale bars = 100 µm.