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. 2023 Apr 21;43(5):493–502. doi: 10.3343/alm.2023.43.5.493

Table 4.

Comparison of responses based on the size of the laboratory test service

Category Question Institutions carrying out ≥6 tests/day (N=80), N (%) Institutions carrying out ≤5 tests/day (N=61), N (%) P
Specimen bottle Patient information included on the bottle 0.0001
Barcoded 39 (48.8) 10 (16.4)
Handwritten 41 (51.3) 51 (83.6)
Bottle for routine chemistry tests 0.5618
Plain tube 16 (20.0) 10 (16.4)
SST 63 (78.8) 51 (83.6)
Others 1 (1.3) 0 (0.0)
Factors that determine the specimen tube used 0.0325
Referral laboratory supplies the specimen collection tube 61 (76.3) 57 (93.4)
Clinic provides referral laboratory-recommended tubes 13 (16.3) 3 (4.9)
Clinic purchases bottles as they see fit 5 (6.3) 1 (1.6)
Unknown* 1 (1.3) 0 (0.0)
Centrifuge A centrifuge is available at the clinic 0.0497
Yes 62 (77.5) 38 (62.3)
No 18 (22.5) 23 (37.7)
When is centrifugation conducted (if the clinic has an on-site centrifuge) 0.0847
~30 minutes after sampling 27 (33.8) 10 (16.4)
Immediately after sampling 25 (31.3) 16 (26.2)
At a designated time (e.g., once a day in the afternoon) 6 (7.5) 4 (6.6)
Rarely use a centrifuge 2 (2.5) 6 (9.8)
On arrival at the referral laboratory 1 (1.3) 2 (3.3)
Others 1 (1.3) 0 (0.0)
Specimen storage/delivery Specimen storage temperature after centrifugation 0.0468
Refrigerator temperature 48 (81.4) 29 (96.7)
Room temperature 11 (18.6) 1 (3.3)
Specimen storage condition after centrifugation 0.1854
In an SST 44 (62.0) 23 (52.3)
In a plain tube 12 (16.9) 12 (27.3)
Transfer serum into a serum separator 9 (12.7) 6 (13.6)
Transfer serum into a microtube 6 (8.5) 3 (6.8)
Specimen storage temperature without centrifugation 0.2028
Refrigerator temperature 12 (54.5) 23 (74.2)
Room temperature 9 (40.9) 8 (25.8)
Freezing temperature§ 1 (4.5) 0 (0.0)
Specimen storage condition without centrifugation 0.0942ll
Upright in a test tube rack 19 (86.4) 22 (71.0)
Placed upright or tilted in paper cups, etc. 2 (9.1) 6 (19.4)
Storage in a basket, etc. 0 (0.0) 3 (9.7)
Unknown 1 (4.5) 0 (0.0)
Whether a guide to specimen storage has been received from the referral laboratory 0.0248
Received 50 (62.5) 26 (42.6)
Not received 30 (37.5) 34 (55.7)
Unknown 0 (0.0) 1 (1.6)
Specimen quality Frequency of experiencing hemolysis that causes erroneous results
~1/10 patients 2 (2.5) 2 (3.3) 0.1786**
~1/100 patients 11 (13.8) 15 (24.6)
~1/1,000 patients 38 (47.5) 15 (24.6)
~1/10,000 patients 20 (25.0) 21 (34.4)
Almost never 9 (11.3) 8 (13.1)
If hemolysis is believed to have caused an erroneous result, the following actions are taken 0.2554††
Resampling and retesting 50 (62.5) 29 (47.5)
If the test is not critical, no further action is taken 22 (27.5) 20 (32.8)
Inform the patient that no resampling will be conducted 13 (16.3) 13 (21.3)
Retest using the previous specimen 15 (18.8) 5 (8.2)
Do not disclose to the patient; no resampling done 2 (2.5) 2 (3.3)
Others 0 (0.0) 3 (4.9)

*Excluded from analysis; Centrifugation after clotting vs. others; In plain tubes vs. others; §Excluded from analysis; llPlace tube in an upright position in a test tube rack vs. others (“unknown” excluded); No response to question. Excluded from analysis; **More than 1/1,000 patients vs.<1/10,000 patients; Resample and retest vs. others.

Abbreviation: SST, serum separating tube.