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. 2023 May 1;13:7073. doi: 10.1038/s41598-023-32922-9

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cholesterol biosynthesis plays a role in cNCC migration. (A) Cell migration of cNCC cultured in HG, CG, NG, and NG2P conditions in the absence (vehicle) or presence of 10 μM fatostatin or fluvastatin was examined in an Incucyte live-cell analyzer via a scratch-wound assay. n = 4 per group; multiple images per well were collected every hour for 36 h. Panel (B) demonstrates same data in the absence of cholesterol synthesis inhibitors to highlight the effect of glucose availability. Each data point represents means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.