KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-caspase-1_p20, clone D7F10 | Cell Signaling Technology | Cat# 3866; RRID:AB_2069051 |
| Rabbit monoclonal cleaved anti-caspase-1, clone D57A2 | Cell Signaling Technology | Cat# 4199; RRID: AB_1903916 |
| Mouse monoclonal cleaved anti-caspase-1, clone casper-1 | AdipoGen | Cat# AG-20B0042; RRID: AB_2490248 |
| Mouse monoclonal anti-caspase-1, clone 1021323 | R&D Systems | Cat# MAB62152 |
| Mouse monoclonal anti-caspase-1 (p10), clone casper-2 | AdipoGen | Cat# AG-20B-0044-C100; RRID:AB_2490253 |
| Rabbit monoclonal cleaved anti-gasderminD | Abcam | Cat# ab209845; RRID:AB_2783550 |
| Rabbit monoclonal cleaved anti-gasderminD, Clone E7H9G | Cell Signaling Technology | Cat# 36425; RRID:AB_2799099 |
| Rabbit monoclonal anti-gasderminD, clone L60 | Cell Signaling Technology | Cat# 93709; RRID:AB_2800210 |
| Mouse monoclonal anti-NLRP3, clone Cryo-2 | AdipoGen | Cat# AG20B0014; RRID:AB_2885199 |
| Rabbit polyclonal anti-NLRP3, clone D4D8T | Cell Signaling Technology | Cat# 15101; RRID:AB_2722591 |
| Mouse monoclonal anti-GFP, clone B-2 | Santa Cruz | Cat# SC9996; RRID:AB_627695 |
| Rabbit monoclonal anti-GFP, clone D5.1 | Cell Signaling Technology | Cat# 2956; RRID:AB_1196615 |
| Rabbit polyclonal anti-ASC, clone AL177 | AdipoGen | Cat# AG25B0006; RRID:AB_2490440 |
| Mouse monoclonal anti-β-tubulin, clone DSHB, AA12.1 | Developmental Studies Hybridoma Bank | Cat# AA12.1 |
| Rabbit polyclonal anti-IL-1β | Santa Cruz | Cat# SC7884; RRID:AB_2124476 |
| Rabbit monoclonal cleaved anti-IL-1b, clone D3A3Z | Cell Signaling Technology | Cat# 83186; RRID: AB_2800010 |
| Rabbit monoclonal anti-IL-1β | Cell Signaling Technology | Cat# 52719; RRID:AB_2799421 |
| Mouse monoclonal anti-IL-1β | Cell Signaling Technology | Cat# 12242; RRID:AB_2715503 |
| HRP conjugated secondary anti-rabbit | Invitrogen | Cat# 31460 |
| HRP conjugated secondary anti-mouse | Invitrogen | Cat# 32430 |
| HRP conjugated secondary anti-goat | Invitrogen | Cat# A15999 |
| Rabbit monoclonal cleaved anti- caspase-3, clone 5A1E | Cell Signaling Technology | Cat# 9664; RRID:AB_2070042 |
| Rabbit monoclonal anti-caspase-3, clone D3R6Y | Cell Signaling Technology | Cat# 14220; RRID:AB_2798429 |
| Anti-Caspase-8 | Cell Signaling Technology | Cat# 4927; RRID:AB_2068301 |
| Rabbit monoclonal cleaved caspase-8, clone D5B2 | Cell signaling Technology | Cat# 8592; RRID:AB_10891784 |
| Anti-caspase-9 | Cell Signaling Technology | Cat# 9504; RRID:AB_2275591 |
| Anti-cleaved caspase-9 | Cell signaling Technology | Cat# 9509; RRID:AB_2073476 |
| Mouse monoclonal cleaved anti-PARP | Cell signaling Technology | Cat# 9548; RRID:AB_2160592 |
| cleaved anti-Caspase-3 (Asp175) | Cell Signaling Technology | Cat# 9661; RRID:AB_2341188 |
| Rabbit monoclonal cleaved PARP (Asp214) | Cell Signaling Technology | Cat#5625; RRID:AB_10699459 |
| Anti-mouse CD45.2-BV570, clone 104 | Biolegend | Cat# 109833 |
| Anti-mouse Ly6G-BV785, clone 1A8 | Biolegend | Cat#127645 |
| Anti-mouse CD3e-BV785, clone 145-2C11 | Biolegend | Cat# 100355 |
| Anti-mouse CD19-BV785, clone 6D5 | Biolegend | Cat# 115543 |
| Anti-mouse NK-1.1-BV785, clone PK136 | Biolegend | Cat# 108749 |
| PE-CF594 Rat anti-mouse SiglecF, clone E50-2440 | BD Biosciences | Cat# 562757 |
| BB700 Rat Anti-Cd11b, clone M1/70 | BD Biosciences | Cat# 566417 |
| Alexa Fluor 700 anti-mouse Cd11c, clone N418 | Biolegend | Cat# 117320 |
| APC anti-mouse Cd11c, clone N418 | Biolegend | Cat# 117310 |
| PE-Cy7 anti-mouse F4/80, clone BM8 | Biolegend | Cat# 123114 |
| APC-Cy7 anti-mouse Ly6C, clone HK1.4 | Biolegend | Cat# 128026 |
| BV421 anti-mouse I-A/I-E, clone M5/114.15.2 | Biolegend | Cat# 107632 |
| BV650 anti-mouse H-2Kb/H-2Db, Clone 28-8-6 | BD Biosciences | Cat# 745287 |
| APC anti-mouse CD115, clone AFS98 | eBioscience | Cat# 17-1152-82 |
| APC/Fire750 anti-mouse H-2Kb/H-2Db | Biolegend | Cat# 114618 |
| Purified anti-mouse CD16/32, clone 93 | Biolegend | Cat# 101302 |
| Mouse Monoclonal anti-IL-18, clone 74 | MBL | Cat# D047-3;RRID:AB_592016 |
| Mouse Monoclonal anti-IL-18, Biotin conjugated | MBL | Cat# D048-6;RRID:AB_592021 |
| Chemicals, peptides, and recombinant proteins | ||
| Fetal bovine serum | Thermo Scientific | Cat# 16140071 |
| Penicillin and Streptomycin | Gibco | Cat# 15140122 |
| DPBS | Corning | Cat# 21-031-CV |
| RPMI | Cytiva Hyclone, Fisher Scientific | Cat# SH30027FS |
| DMEM | Corning | Cat# 10-013-CV |
| Opti-MEM | Gibco | Cat# 31985088 |
| LPS | Invivogen | Cat# Tlrl-3pelps |
| Pam3csk4 | Invivogen | Cat# Tlrl-pms |
| MSU | Invivogen | Cat# Tlrl-msu |
| Silica/NanoSiO2 | Invivogen | Cat# Tlrl-SiO-2 |
| Nigericin | Invivogen | Cat# Tlrl-nig |
| ATP | Sigma-Aldrich | Cat# A6419 |
| CPPD | Invivogen | Cat# Tlrl-cppd |
| Flagellin | Invivogen | Cat# Tlrl-epstfla-5 |
| Lipofectamine | Thermo Scientific | Cat# 11668019 |
| DOTAP | Roche | Cat# 11202375001 |
| PMA | Invitrogen | Cat# J63916.MCR |
| Protease inhibitor | Pierce | Cat# A329631 |
| Trizol | Invitrogen | Cat# 15596026 |
| Protein agarose A/G beads | Santa Cruz | Cat# SC2003 |
| Super Signal West Femto | Thermo Scientific | Cat# 34096 |
| Staurosporin | Sigma-Aldrich | Cat# S6942 |
| WGP dispersible | Invivogen | Cat# Tlrl-wgp |
| Ac-YVAD-AFC | Enzo Life Science | Cat# ALX-260-108-M005 |
| Ac-DEVD-AFC | Enzo Life Science | Cat# ALX-260-032-M001 |
| Ac-IETD-AFC | Enzo Life Science | Cat# ALX-260-110-M005 |
| Ac-LEHD-AFC | Enzo Life science | Cat# ALX-260-116-M005 |
| Biotin-VAD-FMK | Santa Cruz | Cat# Sc311290 |
| Biotin-YVAD-CMK | Anaspec | Cat# AS-60841 |
| Neutravidin | Pierce | Cat# 29200 |
| FSL-1 | Invivogen | Cat# tlrl-fsl |
| Bacillus anthracis lethal factor | List labs | Cat# 104 |
| Protective antigen | List labs | Cat# 104 |
| Poly(dA:dT) | Invivogen | Cat# Tlrl-patn |
| Brilliant Stain Buffer | BD Horizon | Cat# 566349 |
| eBioscience™ Fixable Viability Dye eFluor™ 506 | ThermoFisher | Cat# 65-0866-14 |
| MACS Buffer | Miltenyi | Cat# 130-091-221 |
| FcBlock | BD Biosciences | Cat# 553141 |
| Cytofix/Cytoperm | BD Biosciences | Cat# 554714 |
| Cytotox Red | Essen Bioscience | Cat# 4632 |
| XenoLight Rediject Inflammation probe | Perkin Elmer | Cat# 760536 |
| Luminol sodium salt | Sigma Aldrich | Cat# A4685 |
| FLICA Caspase-1 inhibitor II Biotinylated | Anaspec | Cat# AS-60841 |
| DSS Disuccinimidyl suberate | Thermo Scientific | Cat# A39267 |
| recombinant murine IFN-γ | Peprotech | Cat# 315-05 |
| MISSION ExpressMag magnetic beads | Millipore-Sigma | Cat# SHM03 |
| Puromycin | Santa Cruz Biotechnology | Cat# 53-79-2 |
| Critical commercial assays | ||
| IL-1β ELISA (human) | Invitrogen | Cat# 88-7261-22 |
| IL-1β ELISA (mouse) | Invitrogen | Cat# 88-7013-22 |
| IL-18 ELISA (human) | R&D Systems | Cat# DY318-05 |
| TNF ELISA (human) | Invitrogen | Cat# 887346-76 |
| TNF ELISA (mouse) | Invitrogen | Cat# 88-7324-88 |
| TNF ELISA (human) | R&D Systems | Cat# DY410 |
| TNF ELISA (mouse) | R&D Systems | Cat# DY210 |
| CXCL1 ELISA (mouse) | R&D Systems | Cat# DY453-05 |
| CXCL2 ELISA (mouse) | R&D Systems | Cat# DY452-05 |
| Caspase-1 ELISA (mouse) | Adipogen | Cat# AG-45B-0002-KI01 |
| NLRP3 ELISA (mouse) | LSBio | Cat# LS-F17336-1 |
| GFP Taqman assay | Invitrogen | Cat# Mr04329676_mr |
| CARD16 Taqman assay | Invitrogen | Cat# Hs03008439_s1 |
| CARD17 Taqman assay | Invitrogen | Cat# Hs01910190_s1 |
| CARD18 Taqman assay | Invitrogen | Cat# Hs01043258_m1 |
| MycoAlert Mycoplasma Detection Kit | Lonza | Cat# LT07 |
| LDH Cytotoxicity Assay (CyQUANT) | Invitrogen | Cat# C203021 |
| Verso cDNA Synthesis Kit | Thermo | Cat# 75650200RXN |
| Duolink Proximity Ligation Assay | Millipore Sigma | Cat# DUO92101-1KT |
| Deposited data | ||
| Affymetrix Human Genome U133 Plus 2.0 Array (HG-U133_Plus_2) | (Lauwerys et al., 2015;43 Nzeusseu Toukap et al., 2007)44 | GEO: GSE36700 |
| original, unprocessed western blot images | this manuscript | https://doi.org/10.17632/gz8rc92m38.1 |
| Experimental models: Cell lines | ||
| THP-1 | American Type Culture Collection | Cat# TIB-202 |
| HEK293 | American Type Culture Collection | Cat# CRL-3216 |
| HEK293 lenti-X | Takara Bio | Cat# 632180 |
| L292 | American Type Culture Collection | Cat# CCL-1 |
| CASP1KO THP-1 | (Chu et al., 2018)92 | N/A |
| ASCKD THP-1 | (Bryan et al., 2009)93 | N/A |
| Ctrl GFP THP-1 | this manuscript | N/A |
| GFP-CARD16 THP-1 | this manuscript | N/A |
| GFP-CARD17 THP-1 | this manuscript | N/A |
| GFP-CARD18 THP-1 | this manuscript | N/A |
| ASCKO THP-1 | Invivogen | thp-koascz |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6 | The Jackson Laboratory, bred in house | JAX#:00066; RRID:IMSR_JAX:000664 |
| Mouse: B6.Tg(CD68-CARD16) | this manuscript | N/A |
| Mouse: B6.Tg(CD68-CARD17) | this manuscript | N/A |
| Mouse: B6.Tg(CD68-CARD18) | this manuscript | N/A |
| Mouse: Casp1−/− | V. M. Dixit (Kayagaki et al., 20159) | N/A |
| Oligonucleotides | ||
| Please see Table S1 | N/A | |
| Recombinant DNA | ||
| pCAT-Basic-CARD16 | this manuscript | N/A |
| pCAT-Basic-CARD17 | this manuscript | N/A |
| pCAT-Basic-CARD18 | this manuscript | N/A |
| pLex-GFP-CARD16 | this manuscript | N/A |
| pLex-GFP-CARD17 | this manuscript | N/A |
| pLex-GFP-CARD18 | this manuscript | N/A |
| pCDNA3-caspase-1 CARD mNeonGreen | this manuscript | N/A |
| pCDNA3-caspase-1 CARD mScarlet-I | this manuscript | N/A |
| pCDNA3- mNeonGreen-mScarlet-I | this manuscript | N/A |
| pMD2.G | Addgene | Cat# 12259; RRID:Addgene_12259 |
| psPAX2 | Addgene | Cat# 12260; RRID:Addgene_12260 |
| lentiCRISPRv1 | Addgene | Cat# 49535 |
| Software and algorithms | ||
| FlowJo v10 | BD Biosciences | N/A |
| Cytek acquisition software | Cytek | N/A |
| SkanIt | Thermo | N/A |
| IncuCyte Base Analysis Software | Sartorius | N/A |
| NIS-Elements AR with ai | Nikon | N/A |
| Fiji (Image J) | Schindelin et al., 201294 | N/A |
| Living Image software 4.7.3 | Perkin Elmer | N/A |