Fig. 1.

SARS-CoV-2 infection affects OCLN expression. (A) Vero-E6 cells were infected with rVSV-eGFP-S or SARS-CoV-2-mNG virus at an MOI of 0.1 and then fixed and stained with OCLN, ZO-1, and Claudin-1 antibodies to detect each protein expression and distribution 24 h postinfection by the confocal assay. (B) Vero-E6 cells and A549-hACE2 cells were infected with SARS-CoV-2 at an MOI of 0.01 at indicated different time points. Subsequently, cells were collected, and lysates were prepared for western blot by normalizing to GAPDH. (C) OCLN expression after SARS-CoV-2 infection was quantified by western blot (three independent experiments). (D) OCLN expression in lung tissues of hamsters mock-infected at day 3 (#1, #2, and #3) or infected with SARS-CoV-2 at day 3 (#4, #5, and #6) and day 5 (#7, #8 and #9) postinfection was determined by western blot. (E) OCLN expression was quantified for the results of western blot by normalizing to GAPDH. (F) Vero-E6 cells were infected with rVSV-eGFP-WA-1, rVSV-eGFP-Beta, or rVSV-eGFP-Kappa virus at an MOI of 0.1. Forty-eight hours postinfection, the cells were collected and prepared for western blot. (G) Vero-E6 cells were mock-infected or infected with an UV-inactivated SARS-CoV-2. Cells were fixed, and IFA was performed to detect OCLN. Nuclei were stained by 4′,6-diamidino-2-phenylindole (DAPI).