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. 2023 Apr 17;120(17):e2218623120. doi: 10.1073/pnas.2218623120

Fig. 7.

Fig. 7.

Endosomal entry pathway is involved in OCLN-mediated cell-to-cell fusion. (A–D) Vero-E6 cells were infected with rVSV-eGFP-S and then treated with CatL inhibitor III or Baf-A1, and virus-induced cell-to-cell fusion (A and C) and cell numbers for each syncytium (B and D) (n > 15) were determined. (E–J) Vero-E6 cells transfected with OCLN or control siRNA were infected with SARS-CoV-2-mNG or rVSV-eGFP-S at an MOI of 0.1. After 1-h incubation, virus was removed, and the cell layer overlaid with 3% agarose with or without 5 μg/mL trypsin and Baf-A1 for 72 h. Images of cells (E and H) were captured (scale bars, 300 μm); cell numbers for each plaque (F and I) and virus titer (G, J, K, and L) were determined and analyzed. (M and N) 293FT-hACE2-TMPRSS2 cells transfected with OCLN or control siRNA were infected with SARS-CoV-2-mNG or rVSV-eGFP-S at an MOI of 0.01 for 1 h. After 1-h incubation, virus was removed and overlaid with 3% agarose with Baf-A1. Seventy-two h postinfection, images (M) were captured (scale bars, 300 μm), and cell numbers for each plaque (N) were determined.