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. 2023 Apr 17;120(17):e2215253120. doi: 10.1073/pnas.2215253120

Fig. 5.

Fig. 5.

Characteristics of regenerated HC-like cells. (A) Kinocilia labeled with acetylated tubulin (Ac-TUBA4A) in WT cochlea reprogrammed by the cocktail and infected by ad.Atoh1.mCherry. (B) SLC26A5 (prestin) positive HC-like cells (arrows) in WT cochlea treated with the cocktail and ad.Atoh1.mCherry. (C) The ganglion neurites (labeled with NF-H) were in contact with the immature synaptic ribbons in regenerated HC-like cell (Atoh1-GFP+/PVALB+). (D and E) Low and high magnification pictures delineating the in vivo lineage tracing result. The Tamoxifen-treated adult Sox2-tdTomato mice (4-wk-old) were i.p. injected with Kanamycin/Furosemide (Kana/Furo) to kill hair cells for 7 d, with the subsequent delivery of the cocktail (VLFsiFsiM) into the middle ear space, followed by the injection of Ad.Atoh1-GFP into the inner ear by cochleostomy for 21 d. In the reprogrammed mice, Ad.Atoh1-GFP-treated adult cochleae showed that most regenerated HC-like cells were of SC origin (arrows, ESPN+/GFP+/Sox2-tdT+) in the outer hair cell region (OHCr) from the apex to the midturn. SE: sensory epithelial region.