Fig. 1.

Generation of the most comprehensive human cross-linking mass spectrometry dataset. (A) Experimental overview. (B) Breakdown and summary statistics of the cross-linked unique residue pairs (URP) and the underlying captured unique protein–protein interactions or internally linked proteins. Interprotein refers to cross-linking between peptides mapping to two distinct UniProt accessions, intraprotein to the same accession, and homooligomer to same accession but with overlapping peptide sequences (and hence must derive from two separate molecules). (C) The size and breakdown of annotated subcellular localizations detected in the cross-linked proteome. Note that redundancies are caused by proteins with multiple annotated subcellular assignments.