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. 2023 Apr 18;120(17):e2219418120. doi: 10.1073/pnas.2219418120

Fig. 6.

Fig. 6.

Cross-links support the AlphaFold Multimer-v2 model of the tRNA ligase core complex. DDX1 (Q92499) is in cyan, RTCB (Q9Y3I0) is in magenta, RTRAF (Q9Y224) is in orange and FAM98B (Q52LJ0) is in blue. (A) Rank 1 AF Multimer-v2 model of the tRNA ligase core complex. (B) All five AlphaFold Multimer-v2 models superimposed onto the Rank 1 model using the coiled-coiled core as the reference point. The coiled-coil core comprising of the C-terminal helices of DDX1, RTRAF, and FAM98B together with RTCB is consistent across all five models (red dashed box). (C) Twelve URPs mapped onto the Rank 1 model of the tRNA ligase core complex. Eight URPs are fulfilled (green lines) The remaining four overlength URPs (not shown) were cross-linked to mobile regions of the DDX1 SPRY-RecA domains and the disordered C-terminal tail of RTRAF.