FIGURE 8.

In neurodegenerative conditions (cortex of 5xFAD mice), astrocytes acquire the ability to respond to exogenous NGF by decreasing Ca²⁺ activity in vivo. (A) Imaging of astrocyte Ca²⁺ in 4-month-old WT and 5XFAD mice was performed in awake mice over the motor cortex. (B) Timeline of the experiment. (C) Representative two-photon images of astrocytic activity in WT and 5xFAD before and after NGF (1 μg/ml) administration. (D) Soma peak amplitudes (One-way ANOVA, WT vs. 5xFAD, p < 0.0001; WT vs. 5xFAD + NGF, p < 0.0001; 5xFAD vs. 5xFAD + NGF, p = 0.0262) of the astrocytic Ca²⁺. (E) (One-way ANOVA, WT vs. 5xFAD, p < 0.0001; WT vs. 5xFAD + NGF, p = 0.0044; 5xFAD vs. 5xFAD + NGF, p = 0.0083) of the astrocytic Ca²⁺ (n = 20 cells from three animals per group).