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. 2023 Apr 21;16(4):dmm049942. doi: 10.1242/dmm.049942

Fig. 1.

Fig. 1.

Increased expression of tribbles (trbl) causes motor impairment and decreases survival. (A) Analysis of Trbl expression in larval eye discs. Third instar eye imaginal discs of UAS-trbl-HA flies driven by gmr-Gal4, immunostained for HA (green) and Hoechst (purple). (B) Ubiquitous expression of the UAS-trbl-HA leads to increased trbl transcript levels. mRNA levels were measured by real-time qPCR (mean+s.e.m.; ***P<0.001; unpaired t-test; n=4 biological replicates). (C) Ubiquitous expression of trbl causes a delay in development, with reduced numbers of developed pupae (mean+s.e.m.; **P<0.01; unpaired t-test). (D,E) Ubiquitous expression of trbl leads to transcriptional upregulation of Thor (D) (mean+s.e.m.; ****P<0.0001; unpaired t-test; n=4 biological replicates), but not InR (E) (mean+s.e.m.; NS, not significant; unpaired t-test; n=4 biological replicates). Transcripts were measured in 14-day-old adult males. (F) Ubiquitous expression of trbl decreases motor performance. Adult flies were tested using a standard climbing assay (mean±s.e.m.; *P<0.05; two-way ANOVA with Sidak multiple comparisons test; n≥5 biological replicates). (G) Ubiquitous trbl expression reduces lifespan (log-rank test). Genotypes: in A, w; gmrGal4/UAStrbl-HA;+; in B-G, w; +; daGal4/+ (control), w; UAStrbl-HA/+; daGal4/+ (UAS-trbl).