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. 2023 Apr 24;11(4):e006195. doi: 10.1136/jitc-2022-006195

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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(A) Example multiplex immunofluorescence image of a sample from the exploratory third panel including stains for PCK, DAPI, CD163, CD8, TIM-3, and PD-L1. Image is taken from the tumor–stroma interface. In the magnified region, the triangle indicates a CD8+TIM3 cell, chevron indicates a CD163+ cell, and the arrow indicates a CD8+TIM3+ cell. (B) Spearman’s correlation between interface tumor/CD163 nK(75) and the proportion of CD8+ cells expressing TIM-3. (C) Spearman’s correlation between interface tumor/(CD8-CD163) nK(75) (ie, CD8/CD163 interface co-localization) and the proportion of CD8+ cells expressing TIM-3. (D) Ridgeline distribution density plots demonstrating the proportion of CD8+ cells expressing TIM-3 in the tumor-core, tumor–stroma interface, and stromal zones, stratified by tumor/CD163 nK(75) status. Adjacent boxplots with Wilcoxon p values correspond to the ridgeline density plots. (E) Ridgeline distribution density plots demonstrating the proportion of CD8+ cells expressing TIM-3 in the tumor-core, tumor–stroma interface, and stromal zones, stratified by CD8/CD163 co-localization status. Adjacent boxplots with Wilcoxon p values correspond to the ridgeline density plots. (F) Ridgeline distribution density plots demonstrating PD-L1 density in the tumor-core, tumor–stroma interface, and stromal zones, stratified by Tumor/CD163 nK(75) status. Adjacent boxplots with Wilcoxon p values correspond to the ridgeline density plots. (G) Present cellular clusterin ridgeline distribution density plots demonstrating PD-L1 density in the tumor-core, tumor–stroma interface, and stromal zones, stratified by CD8/CD163 co-localization status. Adjacent boxplots with Wilcoxon p values correspond to the ridgeline density plots. PCK, pan-cytokeratin.