Figure 3.

Effects of Faecalibacterium prausnitzii on the CD4⁺ T cell differentiation of axSpA patient-derived PBMCs. PBMCs (1 x 10⁶ cells) were obtained from axSpA patients (N = 4). These were cultured with Faecalibacterium prausnitzii (1 or 10 μg/mL) with anti-CD3 Ab (0.5 μg/mL) for 72 h. (A) CD4⁺ IL-17A⁺ T cell and CD4⁺ CD25^high Foxp3⁺ T cell differentiation was measured by flow cytometry (presented with representative flow cytometry image). (B) In culture media, the levels of IL-17A and IL-10 were measured by ELISA. ^** P < 0.01, ^*** P < 0.001 and ^**** P < 0.0001.