TABLE 2.
Inhibition of the growth of H. capsulatum yeasts by lysozyme and elastasea
Reagent | Concn (μg/ml) | % Inhibition of growthb |
---|---|---|
Lysozyme | 1 | 16 ± 4 |
10 | 20 ± 5 | |
100 | 36 ± 8 | |
Elastase | 1 | 17 ± 6 |
10 | 22 ± 8 | |
100 | 25 ± 8 |
H. capsulatum yeasts (5 × 103) were incubated with various concentrations of lysozyme and elastase for 24 h at 37°C. The yeasts then were pulsed with [3H]leucine for an additional 24 h to quantitate the growth of the remaining yeasts.
Mean ± SEM (n = 10 for lysozyme and 6 for elastase). The data were not statistically significantly different from the controls (Student's t test).