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Overview of ECM proteomics workflows. Current ECM proteomics workflows can be divided into 5 broad steps: 1) ECM protein enrichment, 2) protein solubilization, 3) protein digestion into peptides, 4) LC-MS/MS acquisition, and 5) database search. Schematics presented in panel 1 are adapted from the template “A Closer Look Into the Extracellular Matrix (ECM) Proteome” by BioRender.com (2022). ECM, extracellular matrix.
