Figure 3. GMF accelerates the detachment of SPIN90‐Arp2/3‐nucleated actin filaments.

1. The fraction of filaments still attached to the surface by SPIN90‐Arp2/3, over time, when exposed to different concentrations of GMF. For each experiment, n = 36–43 filaments. Black lines are exponential fits. Each experiment was repeated three times (technical replicates), yielding similar results.
2. Detachment rates, determined by exponential fits of survival curves shown in (A), as a function of the concentration of GMF. The error bars are determined by fitting the edges of the 95% confidence intervals (not shown, for readability) of the survival curves. The data are fitted by a Michaelis–Menten equation, yielding an apparent dissociation constant K_D = 70 ± 23 nM and a maximum detachment rate of 0.017 ± 0.001 s⁻¹.

Source data are available online for this figure.