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Normalized number of filaments nucleated over time, from SPIN90‐Arp2/3 exposed to 3 μΜ G‐actin (15% labeled with Alexa488) and 3.5 μΜ profilin, with 0 or 50 nM of cortactin. Solid lines are exponential fits, yielding nucleation rates knuc = (2.0 ± 0.04) × 10−3 s−1 without cortactin, and knuc = (5.3 ± 0.08) × 10−3 s−1 with cortactin. Indicated values of n are the number of filaments observed in each experiment.
