Figure 6. Aging destabilizes branches but has no measurable effect on SPIN90‐Arp2/3‐nucleated filaments.

1. Fraction of remaining branches, over time, starting at an average of 5 and 25 min after branch nucleation, in the presence (blue) or absence (gray) of VCA. Lines are exponential fits, yielding debranching rates of (3.7 ± 0.6) × 10⁻⁴ s⁻¹ and (20 ± 8) × 10⁻⁴ s⁻¹ without VCA for 5‐ and 25‐min‐old branches, respectively; and debranching rates of (3.8 ± 1.3) × 10⁻³ s⁻¹ and (8.2 ± 2.8) × 10⁻³ s⁻¹ with 0.5 μM VCA for 5‐ and 25‐min‐old branches, respectively.
2. Fraction of attached SPIN90‐nucleated filaments, over time, starting at an average of 5 and 25 min after branch nucleation, in the presence (blue) or absence (gray) of VCA. Lines are exponential fits, yielding detachment rates of (12 ± 7) × 10⁻⁴ s⁻¹ and (7 ± 3) × 10⁻⁴ s⁻¹ without VCA for 5‐ and 25‐min‐old filaments, respectively (P = 0.2, log‐rank test); and detachment rates of (2.5 ± 1.1) × 10⁻² s⁻¹ and (3.1 ± 1.7) × 10⁻² s⁻¹ with 1 μM VCA for 5‐ and 25‐min‐old filaments, respectively (P = 0.4, log‐rank test).

Data information: Shaded areas represent 95% confidence intervals. Each curve is the result of a single experiment. The experiments on the aging of SPIN90‐Arp2/3 (B) were repeated twice, yielding the same result. The flowing solutions applied a weak force to the filaments (< 1 pN) and to the branches (0.2 pN).

Source data are available online for this figure.