Figure 8. DCP1 Phosphostatus regulates Actin remodeling at the edge.

1. Representative confocal 3D rendering micrographs of root meristematic cells from the WT or the dcp1‐3 mutant stained with phalloidin for actin visualization (N, biological replicates = 3, n = 4). Scale bars: 5 μm (z‐scale is 4 μm). Upper middle: the “Spectrum” micrographs indicate the maximum color‐coded signal intensity (scale on the right, middle inset). Note that the signal is evenly distributed in region 1 (left upper micrograph), whereas it mostly accumulates at the edge or vertex in regions 2 and 3 (see arrowheads; images on top). Lower middle: a detail of the higher actin accumulation at edges/vertices in region 2 (compare regions 1 and 2, Scale bars: 7 μm). Insets (details, Scale bars: 2 μm) indicate the loss of vertex actin accumulation in dcp1‐3. Right: plot profile from the actin signal in the WT or dcp1‐3. The vertices are indicated.
2. Representative confocal micrographs showing actin localization in WT, dcp1‐1, dcp1‐3, scar1234, dcp2‐1, dcp5, and pat1 upon phalloidin staining and graph (right) indicating the percentage of cells in region 3 with an accumulation of actin at edges in various genotypes (N, biological replicates = 3, n = 7–9 roots, bars show means + s.d.). Scale bars: 7 μm.

Data information: In A, P values were determined by Kruskal–Wallis, while in (B), the exact P values were determined by Brown–Forsythe and Welch ANOVA.

Source data are available online for this figure.