Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
One Shot® ccdB Survival™ 2 T1R Competent Cells	ThermoFisher Scientific	A10460
Subcloning Efficiency DH5alpha chemically competent cells	ThermoFisher Scientific	18265‐017
NEB® 10‐beta Competent E. coli	New England BioLabs	C3019I
NEB® 5‐alpha F'Iq Competent E. coli (High Efficiency)	New England BioLabs	C2992I
Agrobacterium tumefaciens GV3101	N/A	N/A
Arabidopsis thaliana Columbia	35Spro:sGFP‐TurboID‐HF/WT	Arora et al (2020)
Arabidopsis thaliana Columbia	35Spro:DCP1‐TurboID‐HF/WT	This paper
Arabidopsis thaliana Columbia	35Spro:GFP‐DCP1/WT	Gutierrez‐Beltran et al (2015)
Arabidopsis thaliana Columbia	DCP1pro:DCP1‐GFP/dcp1‐1	Yu et al (2019)
Arabidopsis thaliana Columbia	DCP1pro:DCP1S237A‐GFP/dcp1‐1	Yu et al (2019)
Arabidopsis thaliana Columbia	DCP1pro:DCP1S237D‐GFP/dcp1‐1	Yu et al (2019)
Arabidopsis thaliana Columbia	RPS5apro:Arpc5‐tagRFP/DCP1‐GFP	This paper
Arabidopsis thaliana Columbia	RPS5apro:Arpc5‐tagRFP/DCP1S237A‐GFP	This paper
Arabidopsis thaliana Columbia	RPS5apro:Arpc5‐tagRFP/DCP1S237D‐GFP	This paper
Arabidopsis thaliana Columbia	dcp1‐1	GABI‐844B03 (Hoffmann et al, 2022)
Arabidopsis thaliana Columbia	dcp1‐3	SAIL_377_B10 (Hoffmann et al, 2022)
Arabidopsis thaliana Columbia	dcp2‐1	SALK_000519.52.10.x (Hoffmann et al, 2022)
Arabidopsis thaliana Columbia	dcp5‐1	SALK_008881 (Hoffmann et al, 2022)
Arabidopsis thaliana Columbia	xrn4‐5	SAIL_681_E01 (Hoffmann et al, 2022)
Arabidopsis thaliana Columbia	pat1‐1	SALK_040660 (Roux et al, 2015)
Arabidopsis thaliana Columbia	arpc5	SALK_123936.41.55 (Pratap Sahi et al, 2017)
Arabidopsis thaliana	scar1234	Dyachok et al (2008), Chin et al (2021)
Arabidopsis thaliana Columbia	brk1	Dyachok et al (2008), Chin et al (2021)
Arabidopsis thaliana Columbia	sok1 sok3	CRISPR double mutant/this paper
Arabidopsis thaliana Columbia	SCAR2pro:SCAR2‐mCherry	Chin et al (2021)
Arabidopsis thaliana Columbia	BRK1pro:BRK1‐mRuby3	Chin et al, 2021
Arabidopsis thaliana Columbia	BRK1pro:BRK1‐YFP	Chin et al, 2021
Arabidopsis thaliana Columbia	UBQ10pro:Lifeact‐mRuby	Chin et al, 2021
Arabidopsis thaliana Columbia	UBQ10pro:Lifeact‐mCherry	Chin et al (2021)
Arabidopsis thaliana Columbia	PAT1pro:PAT1‐GFP	Roux et al (2015)
Arabidopsis thaliana Columbia	SOK3pro:SOK3‐YFP	van Dop et al (2020)
Arabidopsis thaliana Columbia	35Spro:DCP2‐YFP	Jang et al (2019)
Arabidopsis thaliana Columbia	VCSpro:VCS‐GFP	Roux et al (2015)
Arabidopsis thaliana Columbia	UBQ10pro:DCP5‐GFP	Chicois et al (2018)
Arabidopsis thaliana Columbia	DEX˃RAB‐A2cDN	Kirchhelle et al (2016)
Arabidopsis thaliana Columbia	RAB‐A5cpro:RAB‐A5c	Kirchhelle et al (2016)
Arabidopsis thaliana Columbia	UBQ10pro:EosFP‐DCP1	This paper
Arabidopsis thaliana Columbia	PIN2pro:PIN2‐GFP	Xu & Scheres (2005)
Arabidopsis thaliana Columbia	PIN7pro:PIN7‐GFP	Zhou et al (2010)
Recombinant DNA
pICSL86900‐OD	Addgene	86178
pICSL13002	Addgene	50266
pICH47751	Addgene	48002
pICH41414	Addgene	50337
pGWB560	Nakagawa et al (2007)
pG5 (RPS5apro:HF‐GW‐tagRFP)	This paper
pIF1 (RPS5apro:HF‐mScarlet‐GW)	This paper
pIF22 (RPS5apro:mNeon‐GW)	This paper
Codon‐optimized TurboID	Arora et al (2020)
35Spro:sGFP‐TurboID‐HF/WT	Arora et al (2020)
35Spro:DCP1‐TurboID‐HF/WT	This paper
35Spro:GFP‐DCP1/WT	Gutierrez‐Beltran et al (2015)
RPS5apro:ARPC5‐tagRFP	This paper
RPS5apro:mNeon‐ARPC5	This paper
pUbiCAS9‐Red	Durr et al (2018)
pSITE17 (nYFP‐GW)	Chakrabarty et al (2007)
pSITE18 (cYFP‐GW)	Chakrabarty et al (2007)
pSITE18 (nYFP‐GW)‐DCP1	This paper
pSITE17 (cYFP‐GW)‐AT1G33050	This paper
pSITE17 (cYFP‐GW)‐AT2G26920	This paper
pSITE17 (cYFP‐GW)‐RH12	This paper
pSITE17 (cYFP‐GW)‐FLXL1	This paper
pSITE17 (cYFP‐GW)‐XRN3	This paper
pG5‐ECT6	This paper
pG5‐ECT4	This paper
pG5‐VAP27‐1	This paper
pG5‐AT2G26920	This paper
pG5‐AT1G33050	This paper
pG5‐AT5G53330	This paper
pG5‐FLXL1	This paper
pG5‐MLN51	This paper
pG5‐EIN2	This paper
Antibodies
Mouse α‐DCP1	This paper
Polyclonal mouse α‐PAT1	Roux et al (2015)
Monoclonal α‐FLAG® M2‐Peroxidase (HRP)	Sigma‐Aldrich	A8592
Monoclonal mouse α‐FLAG® M2	Sigma‐Aldrich	F1804
Polyclonal Rabbit α‐Green Fluorescent Protein (GFP)	Millipore	AB10145
α‐streptavidin‐HRP	Sigma‐Aldrich	GERPN1231
Monoclonal Mouse α‐streptavidin	Sigma‐Aldrich	189730
Monoclonal Mouse α‐GFP antibody	Sigma‐Aldrich	SAB2702197
Monoclonal Mouse α‐Red Fluorescent Protein (RFP) (clone RF5R)	Agrisera	AS15 3028
Monoclonal Rat α‐tubulin (YL1/2)	Santa Cruz Biotechnology	sc‐53029
Alexa Fluor® 488 phalloidin	ThermoFisher Scientific	A12379
Rhodamine Red™‐X (RRX) 570 AffiniPure Donkey α‐Mouse IgG (H + L)	Jackson ImmunoResearch	715‐295‐151
Rhodamine (TRITC) AffiniPure Donkey α‐Rat IgG (H+L)	Jackson ImmunoResearch	712‐025‐153
IRDye® 680LT Goat α‐Mouse IgG (H + L)	LI‐COR	925‐68020
IRDye® 800CW Goat α‐Rat IgG (H + L)	LI‐COR	925‐32219
Amersham ECL Rabbit IgG, HRP‐linked whole Ab (from donkey)	Amersham	NA934
Amersham ECL Mouse IgG, HRP‐linked whole Ab (from sheep)	Amersham	NA931
Polyclonal rabbit α‐His
Oligonucleotides and sequence‐based reagents
Primers used for cloning	This study	Appendix Table S1
Primers used for verification of T‐DNA mutants	This study	Appendix Table S2
Primers used for generating CRISPR mutants	This study	Appendix Table S3
Chemicals, enzymes, and other reagents
α‐FLAG® M2 Magnetic Beads	Sigma‐Aldrich	M8823
Dynabeads® M‐280 Streptavidin	Thermo Fisher Scientific	11205D
PD‐10 Desalting Columns	Cytiva	17085101
Glutathione Sepharose 4B	Cytiva	17075601
IgG sepharose	Cytiva	170969‐01
Ni‐NTA Agarose	Qiagen Ab	30210
HisPur™ Cobalt Resin	ThermoFisher Scientific	89964
Duolink® In Situ PLA® Probe α‐Rabbit PLUS Affinity purified Donkey anti‐Rabbit IgG (H + L)	Sigma‐Aldrich	DUO92002‐100RXN
Duolink® In Situ PLA® Probe α‐Mouse MINUS Affinity purified Donkey anti‐Mouse IgG (H + L)	Sigma‐Aldrich	DUO92004‐100RXN
Duolink® In Situ PLA® Probe α‐Goat PLUS Affinity purified Donkey anti‐Goat IgG (H + L)	Sigma‐Aldrich	DUO92003‐100RXN
Duolink® In Situ Detection Reagents Red	Sigma‐Aldrich	DUO92008‐100RXN
Duolink® In Situ Detection Reagents FarRed	Sigma‐Aldrich	DUO92013‐100RXN
Duolink® In Situ Probemaker PLUS	Sigma‐Aldrich	DUO92009‐1KT
Duolink® In Situ Wash Buffers, Fluorescence	Sigma‐Aldrich	DUO82049
Gentamycin sulfate	Saveen Werner AB (Duchefa)	G0124.0005
Ampicillin	Saveen Werner AB (Duchefa)	A0104.0010
Spectinomycin	Saveen Werner AB (Duchefa)	S0188.0005
Rifampicin	Saveen Werner AB (Duchefa)	R0146.0005
Kanamycin	Saveen Werner AB (Duchefa)	K0126.0005
Biotin	Sigma‐Aldrich	B4501
Amiprofos methyl (APM)	Sigma‐Aldrich	3992
Latrunculin B (Lat B)	Sigma‐Aldrich	L5288
Cytochalasin D (Cyt D)	Santa Cruz Biotechnology	sc‐201442
Dithiothreitol (DTT)	ThermoFisher Scientific	R0861
Protease inhibitors cocktail	Sigma‐Aldrich	P9599
PhosSTOP	Roche	4906845001
Propidium Iodide	Sigma‐Aldrich	P4170
IGEPAL CA‐630	Sigma‐Aldrich	I8896
Isopropyl‐b‐D‐1thiogalactopyranoside (IPTG)	Sigma‐Aldrich	I6758
Imidazole	Sigma‐Aldrich	56748
TRIzol® Reagent	ThermoFisher Scientific	15596018
30% Acrylamide/Bis Solution, 29:1	Biorad	161‐0156
4× Laemmli Sample Buffer	Biorad	1610747
RiboLock Rnase Inhibitor	ThermoFisher Scientific	EO0381
DNase I, RNase‐free	ThermoFisher Scientific	EN0521
Mesh	SefarNitex	03‐25/19
VectaShield	Vector Laboratories	H‐1200
FM4‐64	ThermoFisher Scientific	T13320
Cycloheximide (CHX)	Sigma‐Aldrich	C7698
Isoxaben	Sigma‐Aldrich	36138
DynaMag™‐2 Magnet	ThermoFisher Scientific	112321D
Phenol: chloroform: iso‐amyl alcohol (25:24:1)	VWR	136112‐00‐0
Acetosyringone	Sigma‐Aldrich	D134406
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa	ThermoFisher Scientific	26619
Eppendorf 1.5 ml Protein LoBind Microcentrifuge tubes	VWR	525‐0133
Bovine serum albumin (BSA)	Sigma‐Aldrich	A7030
Deionized Formamide	Sigma‐Aldrich	S4117
m‐maleimidobenzoyl‐N‐hydroxysuccinimide ester (MBS)	ThermoFisher Scientific	22311
2‐fluoro‐N‐[2‐(2‐methyl‐1H‐indol‐3‐yl)ethyl]‐benzamide (CK‐666)	Sigma‐Aldrich	SML0006
Phusion™ High‐Fidelity DNA Polymerase & dNTP Mix	ThermoFisher Scientific	F530N
SuperScript III First‐Strand Synthesis System for RT‐PCR	ThermoFisher Scientific	18080‐051
Maxima SYBR Green/Flouorescein qPCR Master Mix	ThermoFisher Scientific	K0243
Gateway® BP Clonase™ II Enzyme Mix	ThermoFisher Scientific	11789‐020
Gateway® LR Clonase® II Enzyme mix	ThermoFisher Scientific	11791020
Colloidal blue staining	ThermoFisher Scientific	LC6025
ECL Prime Western Blotting Detection Reagent	Cytiva	GERPN2232
Software
Cytoscape v3.4.0	http://www.cytoscape.org	Shannon et al (2003)
FIJI	https://fiji.sc/	Schindelin et al (2012)
RootTrace	http://www.plant‐image‐analysis.org/software/roottrace	French et al (2008)
JACoP	https://imagej.nih.gov/ij/plugins/track/jacop.html	Bolte and Cordelieres (2006)
Graphpad Prism v9	https://www.graphpad.com/scientific‐software/prism/	Commercial
Adobe Photoshop 2023	https://www.adobe.com	Commercial
Search Tool for the Retrieval of Interacting Genes/Proteins	https://string‐db.org	Freeware
R v4.4.2	https://www.r‐project.org	Freeware
Other
In Fusion HD Cloning Kit	Clonetech/Takara	638909
SuperSignal Western Blot Enhancer	ThermoFisher Scientific	46641
SuperSignal™ West Femto Maximum Sensitivity Substrate	ThermoFisher Scientific	34094
SP8 LIGHTINING MODE CONFOCAL MICROSCOPE	https://www.leica‐microsystems.com	Commercial
Phire Plant Direct PCR Master Mix	ThermoFisher Scientific	F160S
pENTR™/D‐TOPO™ Cloning Kit	Thermo Fisher Scientific	K240020
GeneJET Plasmid Miniprep Kit	ThermoFisher Scientific	K0503
GeneJET Gel Extraction Kit	ThermoFisher Scientific	K0692
Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer	https://www.thermofisher.com/order/catalog/product/IQLAAEGAAPFADBMBHQ	Commercial