Figure EV1. Intracellular growth of Mtb results in death and lysis of the infected macrophage, rapid extracellular growth on the host‐cell debris, and formation of large extracellular Mtb aggregates.
BMDMs were infected with Mtb Erd‐tdTomato and imaged by time‐lapse microscopy at 1‐ or 2‐h intervals for up to 166 h.
- Growth rate of individual Mtb microcolonies growing inside a macrophage (intra) or on the debris of a lysed macrophage (extra). Black lines indicate median values and interquartile ranges. (n = 96, 47 bacterial microcolonies, respectively)
- Percentage survival over time of infected versus uninfected bystander macrophages. (n = 110 and 102, respectively)
- Representative example of an intracellular Mtb microcolony that after lysis of the host macrophage (Draq7 positive cell) grows on the debris of the dead cell. Scale bar, 10 μm. Representative snapshots from Movie EV2.
Source data are available online for this figure.