Fig. 2.

LSI instrumentation, processing scheme, and data quantification. (a) A typical LSI setup where a computer controls the camera. LAS, laser; AL, aspheric lens; P, polarizer; and Cam, camera. LSI processing pipeline from raw data to blood flow maps using (left) spatial and (right) temporal processing algorithms. (b) Quantitation of LSI data. Choosing an ROI depends on the brain being investigated. Three different types of measurements: (top) NVC in response to hindpaw stimulation with quantitative parameters, slope, and peak response; (middle) CVR in response to ${\mathrm{CO}}_2$ inhalation with quantitative parameters, $t_{50\text{rise}}$, AUC of relative CBF during hypercapnia, maximum change in relative CBF (rCBF), and $t_{50\text{fall}}$; (bottom) CBF pulsatility at rest with peak and trough outlined, showing an amplitude can be measured.