Skip to main content
. 2023 Mar 2;83(9):1426–1442. doi: 10.1158/0008-5472.CAN-22-3000

Figure 1.

Figure 1. Cultured cancer cell lines lack de novo cysteine synthesis capacity. A–C, Analysis of de novo cysteine synthesis in cultured NSCLC, SCLC, PDAC, and HCC cell lines with 13C3-serine tracing. Cell lines were incubated with 13C3-serine containing media for 4 hours, followed by analysis of the fraction labeling in serine (A), cystathionine (B), and cysteine (C). Data are presented as mean ± SD and N = 3 biological replicates for each cell line. D, Immunoblotting for the transsulfuration enzymes CBS and CSE. HSP90 was used for the loading control and HepG2 was used for relative comparison between different membranes. Cth, cystathionine; Cys, cysteine; Ser, serine.

Cultured cancer cell lines lack de novo cysteine synthesis capacity. A–C, Analysis of de novo cysteine synthesis in cultured NSCLC, SCLC, PDAC, and HCC cell lines with 13C3-serine tracing. Cell lines were incubated with 13C3-serine containing media for 4 hours, followed by analysis of the fraction labeling in serine (A), cystathionine (B), and cysteine (C). Data are presented as mean ± SD and N = 3 biological replicates for each cell line. D, Immunoblotting for the transsulfuration enzymes CBS and CSE. HSP90 was used for the loading control and HepG2 was used for relative comparison between different membranes. Cth, cystathionine; Cys, cysteine; Ser, serine.