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. 2023 Mar 2;83(9):1426–1442. doi: 10.1158/0008-5472.CAN-22-3000

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©2023 The Authors; Published by the American Association for Cancer Research

This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.

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Figure 2. Contribution of de novo cysteine synthesis to the cysteine pool varies across healthy mouse tissues. A, Schematic depicting 1-[13C1]-serine infusion and its metabolism via the transsulfuration and glutathione synthesis pathways. B–G, Healthy C57BL/6J mice were infused with 1-[13C1]-serine, followed by analysis of the fraction labeling in serine (B), glycine (C), cystathionine (D), glutathione (E), cysteine (F), and γ-glutamylcysteine (G). For B–G, data are presented as mean ± SD and N = 10 mice (5 male, 5 female). N.D., not detected. H, Fractional contribution of serine to intracellular cysteine synthesis in each tissue from B–G. Cysteine labeling was normalized to the fraction labeling of serine in each tissue. I, Immunoblots of CBS and CSE for each tissue. HSP90 was used for the loading control. αKB, α-ketobutyrate; Cth, cystathionine; Cys, cysteine; Gly, glycine; Glut, glutamate; GSH, glutathione; γ-Glu-Cys, γ-glutamylcysteine; Ser, serine. (A, Created with BioRender.com.) — Contribution of de novo cysteine synthesis to the cysteine pool varies across healthy mouse tissues. A, Schematic depicting 1-[¹³C₁]-serine infusion and its metabolism via the transsulfuration and glutathione synthesis pathways. B–G, Healthy C57BL/6J mice were infused with 1-[¹³C₁]-serine, followed by analysis of the fraction labeling in serine (B), glycine (C), cystathionine (D), glutathione (E), cysteine (F), and γ-glutamylcysteine (G). For B–G, data are presented as mean ± SD and N = 10 mice (5 male, 5 female). N.D., not detected. H, Fractional contribution of serine to intracellular cysteine synthesis in each tissue from B–G. Cysteine labeling was normalized to the fraction labeling of serine in each tissue. I, Immunoblots of CBS and CSE for each tissue. HSP90 was used for the loading control. αKB, α-ketobutyrate; Cth, cystathionine; Cys, cysteine; Gly, glycine; Glut, glutamate; GSH, glutathione; γ-Glu-Cys, γ-glutamylcysteine; Ser, serine. (A, Created with BioRender.com.)