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. 2023 Mar 2;83(9):1426–1442. doi: 10.1158/0008-5472.CAN-22-3000

Figure 3.

Figure 3. Cyst(e)ine supplies the cysteine pool in all tissues. A, Schematic depicting 13C6-cystine infusion and its metabolism to glutathione and taurine. B–G, Healthy C57BL/6J mice were infused with 13C6-cystine, followed by analysis of the fraction labeling in cysteine (B), γ-glutamylcysteine (C), cysteine (D), glutathione (E), hypotaurine (F) and taurine (G). For B–G, data are presented as mean ± SD and N = 5 mice. N.D., not detected. H, Immunoblots of xCT, CDO1, CSAD, ADO, FMO1, GCLC, GCLM, and GSS for each tissue. HSP90 was used for the loading control. αKB, α-ketobutyrate; Cth, cystathionine; Cys, cysteine; Cys2, cystine; γ-Glu-Cys, γ-glutamylcysteine; Glut, glutamate; Gly, glycine; GSH, glutathione; Hcy, homocysteine; Htau, hypotaurine; Tau, taurine. (A, Created with BioRender.com.)

Cyst(e)ine supplies the cysteine pool in all tissues. A, Schematic depicting 13C6-cystine infusion and its metabolism to glutathione and taurine. B–G, Healthy C57BL/6J mice were infused with 13C6-cystine, followed by analysis of the fraction labeling in cysteine (B), γ-glutamylcysteine (C), cysteine (D), glutathione (E), hypotaurine (F) and taurine (G). For B–G, data are presented as mean ± SD and N = 5 mice. N.D., not detected. H, Immunoblots of xCT, CDO1, CSAD, ADO, FMO1, GCLC, GCLM, and GSS for each tissue. HSP90 was used for the loading control. αKB, α-ketobutyrate; Cth, cystathionine; Cys, cysteine; Cys2, cystine; γ-Glu-Cys, γ-glutamylcysteine; Glut, glutamate; Gly, glycine; GSH, glutathione; Hcy, homocysteine; Htau, hypotaurine; Tau, taurine. (A, Created with BioRender.com.)