Table 1.
Materials.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| ADO (Rabbit polyclonal antibody) | OriGene | Catalog no. TA322128; Lot no. D814AA091; RRID: AB_2920786 |
| CBS (D8F2P; Rabbit monoclonal antibody) | Cell Signaling Technology | Catalog no. 14782; Lot no. 1; RRID: AB_2798609 |
| CDO1 (Rabbit polyclonal antibody) | Proteintech | Catalog no. 12589-1-AP; Lot no. 57877; RRID: AB_10638145 |
| CSAD (Rabbit polyclonal antibody) | LSBio (LifeSpan) | Catalog no. LS-C375526; Lot no. 124060; RRID: AB_2801349 |
| CSE (Rabbit polyclonal antibody) | Proteintech | Catalog no. 12217-1-AP; Lot no. 00089191; RRID: AB_2087497 |
| FMO1 (Rabbit polyclonal antibody) | LSBio (LifeSpan) | Catalog no. LS-C346135-50; Lot no. 215780; RRID: AB_2920787 |
| GCLC (Mouse mAb) | Santa Cruz Biotechnology | Catalog no. sc-390811; Lot no. E1917; RRID: AB_2736837 |
| GCLM (Rabbit polyclonal antibody) | GeneTex | Catalog no. GTX114075; Lot no. 40156; RRID: AB_10619535 |
| GSS (Mouse mAb) | Novus | Catalog no. NBP2-03351; Lot no. A01; RRID: AB_2920788 |
| HSP90 (Rabbit polyclonal antibody) | Cell Signaling Technology | Catalog no. 4874; Lot no. 6; RRID: AB_2233307 |
| xCT (Rabbit polyclonal antibody) | Cell Signaling Technology | Catalog no. 98051; Lot no. 1; RRID: AB_2800296 |
| Chemicals, peptides, and recombinant proteins | ||
| Glucose | Thermo Fisher Scientific | Catalog no. D16-500 |
| Glycine | VWR | Catalog no. BP381-1 |
| L-Arginine•HCl | Sigma-Aldrich | Catalog no. A6969-25G |
| L-Aspartic acid | MP Biomedicals | Catalog no. 194633 |
| L-Asparagine | Sigma-Aldrich | Catalog no. A4159-25G |
| L-Cystine•2HCl | Sigma-Aldrich | Catalog no. C6727-25G |
| L-Glutamic acid | Sigma-Aldrich | Catalog no. G8415-100G |
| L-Glutamine | VWR | Catalog no. 02-0131-0100 |
| L-Histidine•HCl•H2O | Sigma-Aldrich | Catalog no. H5659-25G |
| Hydroxy-L-proline | TCI | Catalog no. H0296 |
| L-Isoleucine | Alfa Aesar | Catalog no. J63045 |
| L-Leucine | Sigma-Aldrich | Catalog no. L8912-25G |
| L-Lysine•HCl | Sigma-Aldrich | Catalog no. L8662-25G |
| L-Methionine | Sigma-Aldrich | Catalog no. M5308-25G |
| L-Phenylalanine | Sigma-Aldrich | Catalog no. P5482-25G |
| L-Proline | Sigma-Aldrich | Catalog no. P5607-25G |
| L-Serine | Sigma-Aldrich | Catalog no. S4311-25G |
| L-Threonine | VWR | Catalog no. E808-25G |
| L-Tryptophan | Sigma-Aldrich | Catalog no. T8941-25G |
| L-Tyrosine•2Na•2H2O | Sigma-Aldrich | Catalog no. T1145-25G |
| L-Valine | Sigma-Aldrich | Catalog no. V0513-25G |
| [13C3]-serine | Cambridge Isotope Laboratories | Catalog no. CLM-1574-H-0.1 |
| [1-13C1]-serine | Cambridge Isotope Laboratories | Catalog no. CLM-1573-0.25 |
| [13C3]-cysteine | Cambridge Isotope Laboratories | Catalog no. CLM-4320-H-PK |
| Methanol (LC/MS grade) | Thermo Fisher Scientific | Catalog no. 047192.M1 |
| H2O | Honeywell Burdick & Jackson | Catalog no. LC365-4 |
| Acetonitrile | Honeywell Burdick & Jackson | Catalog no. 34998-2.5L |
| N-ethylmaleimide (NEM) | Alfa Aesar | Catalog no. 40526-06 |
| UltraPure Distilled Water | Invitrogen | Catalog no. 10977-015 |
| Ammonium formate | Frontier Scientific | Catalog no. JK967458 |
| DL-Propargylglycine | Fisher Scientific | Catalog no. 437320010 |
| Experimental models: Cell lines | ||
| A549 | ATCC | Catalog no. CCL-185; RRID: CVCL_0023 |
| Calu3 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_0609 |
| H1299 | ATCC | Catalog no. CRL-5803; RRID: CVCL_0600 |
| H1581 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_1479 |
| H1792 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_1495 |
| H1944 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_1508 |
| H1975 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_1511 |
| H1993 | ATCC | Catalog no. CRL-5909; RRID: CVCL_1512 |
| H2009 | ATCC | Catalog no. CRL-5911; RRID: CVCL_1514 |
| H2172 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_1537 |
| H2347 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_1550 |
| H460 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_0459 |
| HCC15 | Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center) | RRID: CVCL_2057 |
| DMS79 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1178 |
| H211 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1529 |
| H526 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1569 |
| 16HC | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_X025 |
| 16HV | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_X026 |
| 86M1 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_8263 |
| H146 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1473 |
| H1838 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1499 |
| H209 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1525 |
| H2107 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1527 |
| H524 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1568 |
| H740 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1586 |
| H82 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1591 |
| H841 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1595 |
| HCC33 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_2058 |
| N417 | Moffitt Lung Cancer Center of Excellence Cell Line Bank | RRID: CVCL_1602 |
| SW210.5 | Dr. John Cleveland, Moffitt Cancer Center | RRID: CVCL_S185 |
| PATU8902 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_1845 |
| PL45 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_3567 |
| MiaPaca2 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_0428 |
| T3M4 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_4056 |
| 8988T | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_1847 |
| 8902 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_1845 |
| BXPC3 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_5I88 |
| CFPAC | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_1119 |
| MPANC96 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_7165 |
| PANC1 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_0480 |
| SUIT2 | Dr. Lewis C. Cantley, Weill Cornell Medicine | RRID: CVCL_3172 |
| SW1990 | ATCC | Cat# CRL-2172; RRID: CVCL_1723 |
| Alex | Dr. Amaia Lujambio, Icahn School of Medicine | RRID: CVCL_0485 |
| HepG2 | Dr. Amaia Lujambio, Icahn School of Medicine | RRID: CVCL_0027 |
| Hep3B | Dr. Amaia Lujambio, Icahn School of Medicine | RRID: CVCL_0326 |
| SNU398 | Dr. Amaia Lujambio, Icahn School of Medicine | RRID: CVCL_0077 |
| SNU449 | Dr. Amaia Lujambio, Icahn School of Medicine | RRID: CVCL_0454 |
| Experimental models: Organisms/strains | ||
| LSL-KrasG12D (Krastm4Tyj) | JAX | RRID:IMSR_JAX:008179 |
| Trp53flox (Trp53tm1Brn) | JAX | RRID:IMSR_JAX:008462 |
| p48-Cre (Ptf1atm1(cre)Hnak) | Dr. Karen Mann, Moffitt Cancer Center | RRID:IMSR_JAX:023329 |
| LSL-Nrf2D29H (Nfe2l2tm1Gmdn) | (28) | MGI:7327101 |
| Rb1flox/flox; Trp53flox/flox; MycLSL/LSL (Igs2tm1(CAG-Myc*T58A/luc)Wrey Trp53tm1Brn Rb1tm3Tyj) | JAX | RRID:IMSR_JAX:029971 |
| Recombinant DNA | ||
| pT3-EF1A-MYC-IRES-luc | (29) | RRID: Addgene_129775 |
| CMV-SB13 vector | (29) | |
| px330 p53 | (29) | |
| Software and algorithms | ||
| El-Maven | https://www.elucidata.io/el-maven | v 0.10.0 or 0.12.0 |
| GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ | Version 9 |
| IsoCor | https://isocor.readthedocs.io/en/latest/ | Version 1.0 or 2.2.0 |
| Xcalibur | Version 4.0 | |
| Other | ||
| RPMI1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder) | US Biological | Catalog no. R9010-01 |
| DMEM w/ L-Glutamine and Sodium Biscarbonate, w/o Glucose, Serine, Glycine (Powder) | US Biological | Catalog no. D9800-16 |
| Dialyzed FBS (dFBS) | Sigma-Aldrich | Catalog no. F0392 |
| DMEM, 1× (Dulbecco's Modification of Eagle's Medium) with 4.5 g/L glucose, L-glutamine and sodium pyruvate | Corning | Catalog no. 10-013-CV |
| RPMI Medium 1640 (1×) [+] L-Glutamine | Gibco | Catalog no. 11875-093 |
| DPBS w/o Calcium or Magnesium | Lonza | Catalog no. 17-512F |
| FBS | Sigma-Aldrich | Catalog no. F0926 |