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. 2023 Apr 14;14:1125759. doi: 10.3389/fimmu.2023.1125759

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Copyright © 2023 Cao, Shi, Liu, Zuo, Jia, Min, Liu, Chen, Zhou, Li, Yang, Liu, Deng, Chen, Chen, Zhang and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutrophils infiltration and NETs formation in mice brain tissue. (A) Representative immunofluorescence staining of Ly6G-positive neutrophils (red) and H3cit -positive(green) and CD31-labeled blood vessels (white) in the mice brain tissue sections post-CCT 3 days. Nuclei were stained with DAPI (blue). (B) Representative Western blot bands of MPO, PAD4, H3Cit, and H3 and statistical analysis expression level of MPO (C), PAD4 (D), and H3Cit (E) in mice brain tissue (n=6). Data are represented as mean ± SD. *P < 0.05, **P < 0.01.