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. 2023 Apr 14;14:1125759. doi: 10.3389/fimmu.2023.1125759

Figure 3.

Figure 3

Neutrophil depletion ameliorates neurological function and reduces NLRP1-induced neuronal death after TBI. Rotary test (A) and modified neural severity scores (mNSS, B) were used to evaluate the neural function of mice treated with vehicle and Anti-Ly6G compared with the Sham group at 1 d, 3 d, 5 d, 7 d after TBI (n = 12). (C) Hematoxylin and eosin staining and Nissl staining of the cortex around the traumatic area 3 days after TBI(n=6). Count the number of dead cells of Hematoxylin and eosin staining (D) and Nissl staining (E). Scale bar = 100 μm. (F) Representative immunofluorescence images of TUNEL-positive(green) cells and NEUN-positive (red) neurons in the peritraumatic cortex (n=6). Count and compare the number of TUNEL-positive neurons in different groups (G). (H) Representative immunofluorescence images of NLRP1-positive(green) cells and NEUN-positive (red) neurons. Count and compare the number of NLRP1-positive neurons in different groups (I). Nuclei were stained with DAPI (blue). Scale bar = 100 μm. Data are represented as mean ± SD. **P < 0.01, ***P < 0.001 and ****P < 0.0001. compared within two groups. #p < 0.05, ##p < 0.01 and vs vehicle group.