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. 2023 Apr 14;14:1125759. doi: 10.3389/fimmu.2023.1125759

Figure 5.

Figure 5

Inhibition of PAD4 ameliorates neurons’ pyroptosis by inhibiting the STING-IRE1α-NLRP1 pathway. (A) Nissl staining images of the peri-injured brain tissue of mice. Count the number of Nissl-positive cells in the different groups (B). Scale bar = 100 μm. (C) Representative fluorescent double stain images of NEUN-positive neurons with STING-positive cells (green), NLRP1-positive cells (green) and Caspase-1-positive cells (green)in the peritraumatic cortex(n=5). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. Count and analyze the number of double-stained positive cells of NEUN-positive neurons with STING-positive cells (D), NLRP1-positive cells (E) and Caspase-1-positive cells (F) in the different groups. (G) Representative Western blot bands of P-IRE1α, NLRP1, ASC, Caspase-1 p20, N-GSDMD, IL-18, IL-1βand statistical analysis expression level of P-IRE1α (H), NLRP1 (I), ASC (J), Caspase-1 p20 (K), N-GSDMD (L), IL-18 (M), IL-1β (N) in brain tissue of mice post-TBI 3 days (n=6). Rotary test (O) and mNSS (P) were used to evaluate the neural function of mice treated with PBS and Anti-Ly6G compared with the Sham group at 1 d, 3 d, 5 d, 7d after TBI (n = 12). Scale bar = 50 μm. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 compared within two groups. #p < 0.05 and vs CL-amidine+ cGAMP group.