TABLE 2.

Recombinant and native CBPs and their roles in virulence

CBP	Contig no.a	Length of open reading frame (bp)	No. of CBD repeats	Predicted no. of amino acids	Protein sizeb (kDa)		Virulence of mutants defective in production of CBPc
					Recombinant	Native	Nasopharynx	Sepsis
LytB	c4270	1,473	8	678	76	70	↓	N
LytC	c4117	2,034	4	491	62	60–65	↓	N
CbpD	c4374	1,347	4	449	T		↓	N
CbpE	c4139	1,884	10	628	70	70–75	↓	N
CbpF	c4103	1,023	8	341	45	41	N	N
CbpG	c4103	426	2	154	22	30–35	↓	↓
CbpI	c4215	636	5	212	29	—	N	N
CbpJ	c4256	999	7	333	33	38	N	N

^aBased on the February 1998 National Center for Biotechnology Information database. 

^bApproximated based on migration on an SDS-polyacrylamide gradient gel. T, truncated form was expressed; —, the antibody against rCBP does not recognize the native protein. 

^cN, normal activity; arrow, decreased activity.