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. 2023 Feb 1;192(1):170–187. doi: 10.1093/plphys/kiad062

Figure 5.

Figure 5

Interactions between maize SMK11 and the assembly factors of complex IV were identified by Y2H, LUC, and BiFC assays. A) Yeast two-hybrid (Y2H) analysis between SMK11 and the four assembly factors (ZmCOX23, ZmMFDX1, ZmMYB3R-3, and ZmIMP1A) of complex IV. The Y2H Gold strain harboring the indicated bait and prey constructs were spotted on SD-Trp-Leu (DDO) and SD-Trp-Leu-His-Ade (QDO). The interaction between Human P53 and T-antigen was used as a positive control. AD, activating domain; BD, binding domain. B) LCI assays between SMK11 and the four assembly factors of complex IV. The specific combinations used for each interaction are indicated. The fluorescence signal intensity represents their interaction activities. C) BiFC assays between SMK11 and the four assembly factors of complex IV. Scale bar =50 μm.