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. 2023 Feb 1;192(1):170–187. doi: 10.1093/plphys/kiad062

Figure 6.

Figure 6

Interactions between maize assembly factors and subunits of complex IV were identified by Y2H assay and BiFC assay. A) Yeast two-hybrid (Y2H) analysis between the four assembly factors (ZmCOX23, ZmMFDX1, ZmMYB3R-3 and ZmIMP1A) and the four subunits (ZmCOX5b-3, ZmCOX6a, ZmCOX-X1, and ZmCOX-X2) of complex IV. The Y2H Gold strain harboring the indicated bait and prey constructs were spotted on SD-Trp-Leu (DDO) and SD-Trp-Leu-His-Ade (QDO). The interaction between the four assembly factors and AD was used as a negative control. AD, activating domain; BD, binding domain. B) BiFC assay between ZmMYB3R-3 and ZmCOX6a. C) BiFC assays between ZmMFDX1 and ZmCOX6a, ZmCOX-X1, as well as ZmCOX-X3. Scale bar =50 μm.