Table 1.
Mass spectrometry analysis of mitochondrial complex IV in smk11 mutant at 15 DAP
| Gene ID | Description | Repeat I | Repeat II | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | smk11 | WT | smk11 | ||||||||||
| Coverage | Unique Peptides | PSM | Coverage | Unique Peptides | PSM | Coverage | Unique Peptides | PSM | Coverage | Unique Peptides | PSM | ||
| GRMZM2G145396 | ZmCOX-X2 | 38.46 | 3 | 6 | 11.54 | 1 | 1 | 44.87 | 4 | 7 | 14.82 | 1 | 1 |
| GRMZM2G134738 | ZmCOX5b-3 | 23.64 | 4 | 8 | 7.27 | 1 | 1 | 64.24 | 7 | 24 | 56.18 | 3 | 7 |
| GRMZM2G177432 | ZmCOX6a | 13.83 | 1 | 1 | — | — | — | 16.26 | 1 | 3 | — | — | — |
| GRMZM2G047512 | ZmCOX-X3 | 7.61 | 1 | 1 | — | — | — | 7.61 | 1 | 2 | — | — | — |
| GRMZM2G159691 | ZmCOX-X1 | 7.34 | 1 | 1 | — | — | — | 14.28 | 2 | 4 | — | — | — |
| GRMZM2G152925 | ZmCOX6b-1 | 5.81 | 1 | 1 | — | — | — | 15.70 | 3 | 3 | — | — | — |
The "-" in Table 1 indicates the proteins were unidentified in the LC-MS/MS analysis.
About 100 μg mitochondrial protein of WT and smk11 was loaded in each lane. BN-PAGE was performed and the gel was stained with CBB. The proteins at the position of mitochondrial complex IV were cut out and used for LC-MS/MS analysis by Shanghai Applied Protein Technology. Coverage represents the percentage of the protein sequence covered by identified peptides. Unique Peptides stand for the number of unique peptide sequences in the protein group. PSM (peptide spectrum matches) stands for the total number of identified peptide sequences for the protein, including those redundantly identified.