Table 2.
Antioxidant enzymes activities in thalli from control and manganese excess and deficiency conditions
| Parameter | Experimental condition | ||||
|---|---|---|---|---|---|
| Ctrl | 30× | 200× | Starch + Mn | Starch − Mn | |
| SOD activity | 3.01 ± 0.63 | 4.19** ± 0.86 | 6.00*** ± 1.48 | 5.63 ± 1.43 | 5.08 ± 0.63 |
| CAT activity | 1.18 ± 0.16 | 0.87** ± 0.26 | 0.54*** ± 0.12 | 0.72 ± 0.16 | 0.70 ± 0.13 |
| PRX activity | 1.27 ± 0.38 | 1.17 ± 0.25 | 1.59* ± 0.42 | 1.52 ± 0.20 | 1.04** ± 0.40 |
| ROS | — | — | — | 44.4 ± 4.2 | 100 ± 1.4 |
Superoxide dismutase, Catalase, and peroxidase activities (µmol substrate mg proteins−1 min−1) were measured in crude extracts. •OH derived from H2O2/O2●− was measured using electron paramagnetic resonance (EPR) as a hydroxyethyl-N-tert-butyl-α-(4-pyridyl)nitrone N′-oxide.
(4-POBN) adduct. Mean value and SD are given. Stars indicate a significant difference compared to the control condition, based on a Mann–Whitney test (*P < 0.01, **P < 0.01, ***P < 0.001, N = 3 biological replicates).