Fig. 1.

NET levels were higher in tumour tissue samples and correlated with poor prognosis in GC patients. A, Representative images of NETs in tumour tissue samples and nontumor tissue samples from the same GC patients detected with MPO and citH3 staining. The white arrows indicate bona fide NETs. Magnification: 20 × . Scale bars: 50 μm. B, The levels of MPO-DNA in the circulation of GC patients and healthy individuals were measured by ELISA. C, NETs in tumour tissue samples were positively correlated with plasma MPO-DNA levels. D and E, GC patients with higher plasma MPO-DNA levels (n = 30) had poorer DFS and OS than those with lower MPO-DNA levels (n = 50). Log-rank test and univariate Cox regression analysis, as indicated. All values are the means ± SDs. ****P < 0.0001