Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 17;14:1148954. doi: 10.3389/fendo.2023.1148954

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Coulter, Greenway, Zhang, Ghosh, Coulter, James, He, Cusimano and Rebello

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Analysis of RXR isoforms bound to immunoprecipitated PPARγ complexes. Adipocytes were treated with vehicle or NRBC for seven days. RXRγ, RXRα and PPARγ protein levels were analyzed by Western blotting. For analysis of whole cell protein levels (input), 50 μg of protein lysate was loaded in each lane. PPARγ was immunoprecipitated from 600 μg of the same whole cell lysate for analysis of PPARγ cofactor binding. An anti-IgGκ light chain secondary antibody was used for detection. This experiment was repeated three times with adipocytes from obese donors.