FIG. 2.
Formation of [3H]IP in J774 cells prelabeled with [3H]inositol after infection with L. monocytogenes strains. IP was extracted from infected cells at 30 min after infection and separated by ion-exchange chromatography as described in the text. The data represent the means ± the SEM for wild-type (n = 12), ΔplcA (n = 4), Δhly (n = 3), ΔplcB (n = 5), and ΔplcA ΔplcB (n = 4) strains. Differences between wild-type and the Δhly, ΔplcA, and ΔplcA ΔplcB strains were all significant (P < 0.01, Mann-Whitney).