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Formation of [3H]IP and [3H]IP3 in murine bone marrow-derived macrophages prelabeled with [3H]inositol after infection with L. monocytogenes. Radioactive products of PI hydrolysis (IP) (A) and PIP2 hydrolysis (IP3) (B) were extracted from infected cells at the times indicated and separated by ion-exchange chromatography as described in the text. The data represent the means ± the SEM of two separate experiments.
